全文获取类型
收费全文 | 1723篇 |
免费 | 211篇 |
出版年
2021年 | 23篇 |
2016年 | 24篇 |
2015年 | 38篇 |
2014年 | 39篇 |
2013年 | 55篇 |
2012年 | 85篇 |
2011年 | 64篇 |
2010年 | 42篇 |
2009年 | 36篇 |
2008年 | 61篇 |
2007年 | 68篇 |
2006年 | 60篇 |
2005年 | 60篇 |
2004年 | 65篇 |
2003年 | 67篇 |
2002年 | 55篇 |
2001年 | 45篇 |
2000年 | 56篇 |
1999年 | 40篇 |
1998年 | 26篇 |
1997年 | 18篇 |
1996年 | 22篇 |
1995年 | 18篇 |
1994年 | 22篇 |
1993年 | 29篇 |
1992年 | 36篇 |
1991年 | 45篇 |
1990年 | 33篇 |
1989年 | 42篇 |
1988年 | 27篇 |
1987年 | 44篇 |
1986年 | 44篇 |
1985年 | 44篇 |
1984年 | 32篇 |
1983年 | 24篇 |
1982年 | 22篇 |
1981年 | 17篇 |
1980年 | 21篇 |
1979年 | 24篇 |
1978年 | 18篇 |
1977年 | 22篇 |
1976年 | 25篇 |
1975年 | 41篇 |
1974年 | 24篇 |
1973年 | 14篇 |
1972年 | 16篇 |
1971年 | 12篇 |
1970年 | 15篇 |
1969年 | 16篇 |
1967年 | 13篇 |
排序方式: 共有1934条查询结果,搜索用时 46 毫秒
21.
22.
23.
24.
25.
26.
Electron microscopy studies have shown that the structure of the complex of myosin subfragment 1 (S-1) cross-linked to actin with 1-ethyl-3-[3-(dimethyl-amino) propyl] carbodiimide is very different in the presence and absence of ATP (Craig, R., Greene, L. E., and Eisenberg, E. (1985) Proc. Natl. Acad. Sci. U. S. A. 82, 3247-3251). More recent studies have found that the structure of the cross-linked complex between S-1 modified extensively with N-ethylmaleimide (NEM.S-1) and actin resembles that of the rigor complex both in the presence and absence of ATP, whereas the structure of the cross-linked complex between S-1 modified with N',N'-p-phenylenedimaleimide (pPDM.S-1) and actin resembles that of the cross-linked actin.S-1 complex in the presence of ATP. In the present study, we have obtained biochemical evidence supporting these results. The conformation of the different cross-linked actin.S-1 complexes was determined by studying their effect on the troponin-tropomyosin-actin complex (regulated actin). The basis of this probe for conformation is that S-1.ATP, which is in the weak-binding conformation, interacts very differently with regulated actin than S-1 or S-1.ADP, which are in the strong-binding conformation. We find that both in the presence and absence of ATP, cross-linked NEM.S-1 appears to be in the strong-binding conformation, whereas cross-linked pPDM.S-1 appears to be shifted toward the weak-binding conformation. In contrast, cross-linked unmodified S-1 appears to be in the strong-binding conformation in the presence of ADP and the weak-binding conformation in the presence of ATP. Therefore, in agreement with electron microscopy studies, the cross-linked actin.S-1 complex appears to be able to alternate between the weak-binding and strong-binding conformation during the cross-bridge cycle. 相似文献
27.
Correigh M. Greene Jason E. Hall Kimberly R. Guilbault Thomas P. Quinn 《Biology letters》2010,6(3):382-386
A principle shared by both economists and ecologists is that a diversified portfolio spreads risk, but this idea has little empirical support in the field of population biology. We found that population growth rates (recruits per spawner) and life-history diversity as measured by variation in freshwater and ocean residency were negatively correlated across short time periods (one to two generations), but positively correlated at longer time periods, in nine Bristol Bay sockeye salmon populations. Further, the relationship between variation in growth rate and life-history diversity was consistently negative. These findings strongly suggest that life-history diversity can both increase production and buffer population fluctuations, particularly over long time periods. Our findings provide new insights into the importance of biocomplexity beyond spatio-temporal aspects of populations, and suggest that maintaining diverse life-history portfolios of populations may be crucial for their resilience to unfavourable conditions like habitat loss and climate change. 相似文献
28.
NGF and other growth factors induce an association between ERK1 and the NGF receptor, gp140prototrk.
As detected by coimmunoprecipitation from PC12 cells, NGF induces rapid association between ERK1 (a growth factor-activated serine/threonine protein kinase) and gp140prototrk NGF receptors. In contrast, no such association is found with the closely related ERK2. Anti-trk immunocomplexes generated from NGF-treated cells also contain protein kinase activity that shares many properties with soluble ERK1. The association of both ERK1 protein and ERK-like kinase activity with gp140prototrk is maximal by 5 min of NGF treatment, persists for approximately 1 hr, and subsequently declines by 18 hr. Treatment with either basic fibroblast growth factor, epidermal growth factor, or orthovanadate also leads to association of ERK1 with gp140prototrk without tyrosine phosphorylation of the latter. The interaction between ERK1 and gp140prototrk may prove relevant to the NGF mechanism. 相似文献
29.
30.